Products

sodium dodecyl binding

The binding of sodium dodecyl sulphate to various proteins

The binding of sodium dodecyl sulphate to proteins by equilibrium dialysis was investigated. 2. Most of the proteins studied bound 90–100% of their weight of sodium dodecyl sulphate. 3. The glycoproteins studied bound 70–100% of their weight of sodium dodecyl sulphate, calculated in terms of the polypeptide moiety of the molecule.

More

Binding of deoxycholate, Triton X-100, sodium dodecyl

Binding of sodium dodecyl suplhate to an integral membrane protein and to a water-soluble enzyme. Journal of Chromatography A 1990, 512, 3-12. DOI: 10.1016/S0021-9673(01)89469-3. Peter A. JEKEL, Henk J. BAK, Nell M. SOETER, Johan M. VEREIJKEN,

More

Sodium Dodecyl Sulfate an overview ScienceDirect Topics

Sodium dodecyl sulfate (SDS), also known as lauryl sulfate, is an ionic detergent that is useful for the rapid disruption of biological membranes. It is a key component of many reagents used to purify nucleic acids because of its abilities to quickly disrupt the tissue architecture and to inhibit both RNase and deoxyribonuclease (DNase) activity.

More

[PDF] Binding of dodecyl sulfate to proteins at high

A wide variety of proteins have been shown to bind identical amounts of an amphiphile, sodium dodecyl sulfate, on a gram per gram basis at monomer equilibrium concentrations above 0.5 mM. The binding is independent of ionic strength and primarily hydrophobic in nature. Only the monomeric form of the amphiphile binds to proteins, not the micellar form.

More

Bindingof Dodecyl SulfatetoProteinsat High Binding Ratios

Binding ratios for bovine serum albumin andhemoglobin were also obtained bydialyz-ing the native protein against SDSand beta-mercaptoethanol thus omitting the GuHCl step. Sodium dodecyl sulfate concentrations in the dialysate and the protein solution were determinedbyextraction of analkyl sulfate-methylene blue complexinto chloroformand

More

Sulfate Columbia University

sodium dodecyl sulfate in bulk solution as detected by surface tension measurements. solution. A binding ratio of 0.67 mol SOS per mol ofeth-ylene oxide on silica was obtained versus 0.38-0.41 for the binding in bulk solution. There was no measurable effect

More

Sodium p-dodecylbenzenesulfonate C18H29NaO3S PubChem

sodium 4-dodecylbenzenesulfonate. Sodium p-dodecylbenzenesulfonate. 2211-98-5. UNII-HB2D2ZEI04. HB2D2ZEI04. Benzenesulfonic acid, 4-dodecyl-, sodium salt (1:1) DODECYLBENZENESODIUMSULFONATE. Benzenesulfonic acid, 4-dodecyl-, sodium salt. Bio-Soft S 101 (sodium) SCHEMBL446403. DTXSID9042413. Sodium 4-dodecylbenzenesulphonate. 4

More

Sodium dodecyl sulfate C12H25NaO4S PubChem

Sodium dodecyl sulfate C12H25NaO4S CID 3423265 structure, chemical names, physical and chemical properties, classification, patents, literature, biological

More

Interactions between gelatin and sodium dodecyl sulphate

The binding of sodium dodecyl sulfate (SDS) and sodium octyl sulfate (SOS) to gelatin and its derivatives at pH 5. 80 has been studied by the method of equilibrium dialysis. The binding has been

More

Binding of deoxycholate, Triton X-100, sodium dodecyl

Binding of sodium dodecyl suplhate to an integral membrane protein and to a water-soluble enzyme. Journal of Chromatography A 1990, 512, 3-12. DOI: 10.1016/S0021-9673(01)89469-3. Peter A. JEKEL, Henk J. BAK, Nell M. SOETER, Johan M. VEREIJKEN,

More

Study of the binding of sodium dodecylsulfate to dibasic

Oct 01, 1994· The impact of sodium chloride and acetic acid was similar, but the effect of sodium acetate was significantly weaker. The strong dependence of the strength of interaction on the salt concentration and on the pH of the eluent indicates that hydrophilic, probably electrostatic forces are involved in the SDS-protein interaction.

More

Binding Isotherms of Sodium Dodecyl Sulfate to Protein

May 01, 1975· To clarify the mode of interaction between sodium dodecyl sulfate (SDS) and protein polypeptides with special reference to SDS-polyacrylamide gel ele We use cookies to enhance your experience on our website.By continuing to use our website, you are agreeing to our use of cookies.

More

[PDF] Binding of dodecyl sulfate to proteins at high

A wide variety of proteins have been shown to bind identical amounts of an amphiphile, sodium dodecyl sulfate, on a gram per gram basis at monomer equilibrium concentrations above 0.5 mM. The binding is independent of ionic strength and primarily hydrophobic in nature. Only the monomeric form of the amphiphile binds to proteins, not the micellar form.

More

Sulfate Columbia University

sodium dodecyl sulfate in bulk solution as detected by surface tension measurements. solution. A binding ratio of 0.67 mol SOS per mol ofeth-ylene oxide on silica was obtained versus 0.38-0.41 for the binding in bulk solution. There was no measurable effect

More

Binding of Sodium Dodecyl Sulfate to Bovine Serum Albumin

Sep 22, 1998· The binding of sodium dodecyl sulfate (SDS) onto this preadsorbed BSA layer was studied at a constant SDS concentration of 1 × 10-4 M with different isotopic compositions of SDS and water. The surface excesses of BSA, SDS, and the structural distributions of BSA, SDS, and water were obtained by simutaneously fitting a single structural model

More

Detergent binding explains anomalous SDS-PAGE migration of

Migration on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) that does not correlate with formula molecular weights, termed “gel shifting,” appears to be common for membrane proteins but has yet to be conclusively explained. In the present work, we investigate the anomalous gel mobility of helical membrane proteins using a library of wild-type and mutant helix-loop

More

The Binding of Detergents to Proteins

The binding of sodium dodecyl sulfate to proteins has been studied since the mid-1940’s (3). A large body of work has ap- peared since then and is summarized in Reference 4. Further- more, two groups have already studied the binding of very high concentrations of

More

Binding of Heavy Metals to Derivatives of Cholesterol and

The binding behaviors of five metals (cadmium, copper, nickel, lead, and zinc), individually at pH 6 and collectively at pHs 6 and 3, to deoxycholic acid (DCA) and taurocholic acid (TCA) were compared with those of sodium dodecyl sulfate (SDS) using a continuous diafiltration method.

More

PRIME PubMed Binding of sodium dodecyl sulfate and

Binding of sodium dodecyl sulfate and hexaethylene glycol mono-n-dodecyl ether to the block copolymer L64: electromotive force, microcalorimetry, surface tension, and small angle neutron scattering investigations of mixed micelles and polymer/micellar surfactant complexes. Langmuir : the ACS Journal of Surfaces and Colloids, 21(22), 10197-208.

More

Binding of Dodecyl Sulfate to Proteins at High Binding

A wide variety of proteins have been shown to bind identical amounts of an amphiphile, sodium dodecyl sulfate, on a gram per gram basis at monomer equilibrium concentrations above 0.5 mM. The binding is independent of ionic strength and primarily hydrophobic in nature. Only the monomeric form of the amphiphile binds to proteins, not the micellar form.

More